Chromatography is one of the commonly used strategies to isolate proteins and peptides for different levels of purities and concentrations.

The various modes of liquid chromatography allow a wide range of flexible biomolecule separations. The different modes of separation rely on specific molecule interaction as the size or MW range of the target biomolecule, polarity/lipophilicity, ionic charge, affinity, or immunoaffinity. Because of the wide usage of different chromatography modes, we wanted to validate the functionality of the KeyView UV detector under several modes and conditions. In this example, the KeyView UV detector was used under three modes of chromatography to validate the compatibility and benefit of solid state UV detection in a protein preparation application...


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